identification of novel mutations in il-2 gene in khorasan native fowls

The intron-exon structure of khorasan native fowl interleukin-2 (il-2) wasinvestigated. for this purpose, twenty chickens were selected from the nativefowl breeding station of khorasan province, and genomic dna was extractedusing a modified conventional dna extraction protocol. an 875 bp fragmentof il-2 was successfully amplified, including a small part of the promoter,exon 1, intron 1, and exon 2. to report a novel mutation in il-2, pcrsequencingtechniques were applied to the gene obtained from khorasan nativefowls. then, the sequences were compared to evaluate gene mutations usingclc main workbench v. 5.5. the i-tasser server was used to predict the 3-dimensional structure and function of protein molecules from amino acidsequences. the results of the comparison showed mutations in the dnafragments of the sample birds, which caused changes in the amino acid contentof il-2. substitutions at points 733, 738, 744, and 794 bp in the sequence werefor c/a, a/t, c/a, and c/t, respectively. the translation of nucleotide toprotein using blastx in the ncbi revealed that nucleotide substitutionscaused three amino acid substitutions including a/e, r/w, and p/t.