protective effects of bacillus sp. mbia2.40 and gallipro on growth performance, immune status, gut morphology and serum biochemistry of broiler chickens feeding by aflatoxin b1

A total of 540 one-day old male broilers ross 308 were allocated to 6treatments in a completely randomized designed with a 2×3 factorialarrangement (aflatoxin b1 (afb1) and probiotics) with 6 replicates and 15birds in each. factors include aflatoxin b1 (control, 500 ppb) and probiotics(control, 10^8 cfu/ml bacillus sp. mbia2.40, 0.2 g gallipro per kg). resultsshowed that adding 500 ppb afb1 to broiler diets resulted in a significantdecrease of body weight gain and feed intake (p < 0.05). the highest of feedconversion ratio was observed in afb1 group (p < 0.05). average bodyweight gain was significantly increased while feed conversion ratio decreasedby bacillus sp. mbia2.40 and gallipro compared with control group (p <0.05). the supplementation of bacillus sp. mbia2.40 and gallipro incontaminated diets relieved the negative effects of aflatoxin on performance.increased serum aspartate amino transferase, alanine amino transferase,alkaline phosphatase and lactate dehydrogenase were observed in dietcontaminated with afb1 compared with other groups (p < 0.05). the lowestantibody production against newcastle disease and sheep blood cells and alsothe lowest skin response to phytohemagglutinin were observed in the afb1group (p < 0.05). villus height and width were significantly increased byprobiotics diets without afb1 in compared with others (p < 0.05). the cryptdepth was significantly higher by birds fed afb1 rather than that others (p <0.05). improving villus height, width and crypt depth by the diets containingprobiotics + afb1 were found as similar as the control diet (p > 0.05). ingeneral, the results of this study showed that adding of bacillus sp. mbia2.40and gallipro in contaminated diets reduce negative effects of afb1 andconsequently these probiotics (especially bacillus sp. mbia2.40) have aprotective effect against aflatoxicosis in broiler chickens.