the the role of eight weeks of combined high-intensity interval training in modulating intrahepatic fndc5 protein and irisin levels in male rats with non-alcoholic steatohepatitis

Background: non-alcoholic steatohepatitis (nash) is one of the prevalent metabolic diseases, and knowing its treatment methods is very important. this study investigates the effect of eight weeks of combined high-intensity interval training on intrahepatic fndc5 protein and irisin in male rats with non-alcoholic steatohepatitis.methods: in this study, 40 rats aged 6 to 8 weeks were divided into two groups: healthy (n=20) and high-fat diet (hfd) (n=20). after eight weeks and assurance of disease induction, the hfd group was randomly divided into control-patient (n=9) and training-patient (n=9). also, the healthy group was divided into control-healthy (n=9) and training-healthy (n=9). the training group rats performed hiit in aquatic and land environments (saturdays and wednesdays in aquatic environments and mondays on a treadmill). western blot method was used to measure fndc5 and irisin proteins, and the spectrophotometric method was used to measure liver enzymes (alt and ast). one-way anova and bonferroni’s post hoc test (p<0.05) were used to determine the difference between groups.results: after eight weeks of combined high-intensity interval training, there was no significant difference in intrahepatic fndc5 protein levels between the groups (p=0.125). intrahepatic irisin protein levels significantly increased in the training-healthy group compared to the control-healthy group (p=0.046). additionally, there was a significant increase in the training-patient group compared to the control-patient group (p=0.036) and a significant increase in the training-healthy group compared to the control-patient group (p=0.011).conclusion: in general, combined high-intensity interval training (aquatic + land) can increase intrahepatic irisin. thus, this type of training can be considered one of the potential non-pharmacological options for treating nas. however, more research is needed to reach definitive results.