effect of eight weeks of high-intensity interval training and moderate-intensity continuous training with quercetin supplementation on the gene expression of foxo1 and atg5 in the liver of diabetic obese rats

Background: the use of complementary medicine in healthcare is increasing rapidly. therefore, the aim of the current study was to investigate the effect of eight weeks of high-intensity interval training and moderate-intensity continuous training with quercetin supplementation on the gene expression of foxo1 and atg5 in the liver of diabetic obese rats.methods: in this experimental study, 42 male wistar rats were considered research samples at eight weeks. rats were induced with diabetes after eight weeks of a high-fat diet and familiarization with a laboratory environment and treadmill. rats were divided into seven groups, and six rats were placed in each group (n=6): healthy control group, diabetes control group, diabetic quercetin group, high-intensity interval training with diabetes group, moderate-intensity continuous training with diabetes group, diabetic high-intensity interval training with quercetin group, and diabetic moderate-intensity continuous training with quercetin group. for one training group, eight weeks of high-intensity interval training, and for the other group, eight weeks of moderate-intensity continuous training on the treadmill were performed. seventy-two hours after the last training session, liver tissues were isolated to check the gene expression of foxo1 and atg5. one-way analysis of variance test was used to check the difference between groups by spss version 26 software.results: findings showed that by inducing type 2 diabetes, gene expression of foxo1 increased (3.14 unit) (p<0.001) and atg5 gene expression decreased (0.71 unit) (p<0.001). after eight weeks of training investigation, gene expression of foxo1 and atg5 decreased (p<0.001) and increased (p<0.001), respectively, in all training groups compared to the diabetic control group. there was no significant difference between the four training groups (p>0/05).conclusion: both exercises with and without quercetin had a modulating effect on the gene expression of indicators related to the process of autophagy and blood glucose levels in the liver of diabetic obese rats.