IN VITRO STERILIZATION PROCEDURES FOR MICROPROPAGATION OF ‘OBLACINSKA’ SOUR CHERRY

Surface sterilization is the most important step in preparation ofexplants for micropropagation, because controlling fungal and bacterialcontamination of woody plant from field sources is very difficult. six sterilizingagents: sodium hypochlorite (naocl), calcium hypochlorite [ca(clo)2], sodiumdichloroisocyanurate (dica), mercuric (ii) chloride (hgcl2), silver nitrate(agno3) and hydrogen peroxide (h2o2) were tested for sterilization of‘obla?inska’ sour cherry buds, by varying their concentration and time ofexposure. the aim of this study was to establish best surface sterilization for invitro propagation of ‘obla?inska’ sour cherry. aseptic cultures of ‘obla?inska’sour cherry were established from axillary buds which were placed in nutrientmedium, supplemented with plants hormones 6-benzylaminopurine (ba), 1-naphthaleneacetic acid (naa) and gibberellic acid (ga3). the results indicatedthat among these sterilizing agents silver nitrate (agno3) at concentration of 1%for 20 minutes was the best for controlling the infection, whereas sterilization withsodium dichloroisocyanurate (dica) at concentration of 1% for 10 minutes wasnot satisfactory.