An Association Analysis of Reelin Gene (RELN) Exon 22 (G/C), Rs.362691, Polymorphism with Autism Spectrum Disorder among Iranian-Azeri Population

Background: autism spectrum disorder (asd) is a intricate childhood neuropsychiatric disorder that is described by deficits in communication of verbal and non-verbal, reciprocal social interactions, stereotypic behaviors, interests, and activities. the studies of post-mortem neuro-anatomical anomalies have indicated that migration alterations could occur early during development (first trimester) in autistic brain. since the reelin gene, plays a crucial role in these migratory processes, it is subsequently considered as a potential candidate gene for autism. materials and methods: in this case-control study, we recruited 74 patients with asd and 88 healthy controls from iranian-azeri population. genomic dna isolated from blood leukocytes of cases and control individuals by the proteinase k and using salt-out method. single nucleotide polymorphisms (snp) genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) technique. results: the allele and genotype frequencies did not show significant difference between autistic and control groups (p>0.05). no significant relationship was observed between the genders and genotypes in autism group (p>0.05). conclusion: the current study showed that the snps rs362691 could not be used as a useful molecular biomarker to predict genetic susceptibility for asd among iranian-azeri patients.