UJI PENGHAMBATAN AKTIVITAS α-GLUKOSIDASE EKSTRAK DAN FRAKSI DAUN Antidesma neurocarpum Miq.

Diabetes mellitus is a metabolic dysfunction disease showed by hyperglicemia and abnormality of carbohydrates, fats, and proteins metabolism. it is known can be cured with a-glucosidase inhibitor. previous experiment reported that methanol extract from leaves of antidesma neurocarpum miq. has a strong inhibitory activity of a-glucosidase (ic50=2.18 μg/ml). the aim of this experiment was to know about the most active extract and fraction of inhibitory activity of a-glycosidase and also the chemical compounds from those most active extract and fraction. extraction is done by using multilevel maceration (n-hexane, ethyl acetate and methanol). inhibitory activity of a-glucosidase tested with microplate reader at 405 nm wavelength. as standard, we used acarbose (ic50=38.37 μg/ml). methanol extract has the strongest inhibitory activity of a-glucosidase as shown the highest inhibitor percentage (89.53%). it is identified the chemical compounds and fracinationed by flash column chromatography. fractination produced 15 combined fractions. combined fractions which more than 200 mg tested to know their inhibitory activity of a-glucosidase. result showed that the most active fraction is the 8th (ic50=40.77μg/ml) with a competitive inhibitor mechanism to a-glycosidase. chemical compounds that is found in the methanol extract and 8th fraction of antidesma neurocarpum miq. leaves areflavonoids, tannins, glycosides, fenol and alkaloids