determination of genetic uniformity in transgenic cotton plants using dna markers (rapd and issr) and sds-page

One concern about using transgenic plants is the un-uniformity that derived from somaclonal variation molecular markers play an important role in the efficient and effective determination of genetic variation. the present work was carried out to assess the genetic uniformity of transgenic cottons (bt and chitinase lines), using rapd, issr molecular markers and sds-page analysis. similarity matrix for rapd marker based on nei and li’s coefficient revealed that p-values for pairwise comparisons between bt and chitinase cottons and their conventional counterpart plant were 93% and 95% respectively. also, similarity matrix for issr marker revealed that p-values for pairwise comparisons between bt and chitinase lines and their conventional counterpart were 94% and 96% respectively. pairwise similarity coefficient among two transgenic plants and their conventional counterparts ranged from 0.94 to 0.99 sds-protein patterns exhibiting four major bands. also, molecular weight ranged from 10 kd to 35 kd. similarity matrix for protein patterns revealed that pairwise value between bt and chitinase lines and their conventional counterpart was 100%. all protein bands of three cultivars were the same and no polymorphic bands were detected. pairwise similarity coefficient among two transgenic plants and their conventional counterpart ranged from 0.94 to 0.99. these lines could be used for more in-depth evaluation and new ways of commercializing biosafety studies can be proposed.