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   the effect of different concentrations of tdz and ba on in vitro regeneration of iranian cannabis (cannabis sativa) using cotyledon and epicotyl explants  
   
نویسنده movahedi mehdi ,ghasemi-omran valiollah ,torabi sepide
منبع journal of plant molecular breeding - 2015 - دوره : 3 - شماره : 2 - صفحه:20 -27
چکیده    The present study was carried out to investigate micropropagation possibility and determine the optimal medium composition and plant growth regulators (pgrs) combinations under in vitro conditions in iranian cannabis. the cotyledon and epicotyl explants obtained from 1 month old in vitro grown seedlings were used in ms medium containing ba (0.1, 0.2, 0.5, 1, 2 and 3 mg^-1) and tdz (0.1, 0.2, 0.5, 1, 2 and 3 mg^-1) either alone or in combination with 0.5 mg^-1 iba. callus formation had priority over direct regeneration in most of the pgrs treatments. comparing the two explants, cotyledon had higher callus formation frequency and the largest callus volume was obtained for this explant in ms medium supplemented with 3 mg^-1 tdz + 0.5 mg^-1 iba. the highest callus fresh weight (3.15 gr) was obtained for cotyledon explant treated with 2 mg^-1 tdz+ 0.5 mg^-1 iba. in shoot formation step, the highest rate of shoot regeneration was achieved in the calli produced from epicotyl explant treated with 2 mg^-1 ba + 0.5 mg^-1 iba; and the highest length of regenerated shoots (1.23 cm) was observed in 2 mg^-1 ba + 0.5 mg^-1 iba treatment. in general, cotyledon was the best explant and tdz in combination with iba was the best treatment for callus formation. epicotyl explant also showed better regeneration compared to cotyledon.
کلیدواژه cannabis ,callus ,plant growth regulators ,regeneration
آدرس islamic azad university, science and research branch, ایران, sari agricaltural sciences and natural resources university, genetics and agricaltural biotechnology institute of tabarestan, ایران, islamic azad university, science and research branch, department of plant breeding and biotechnology, ایران
 
     
   
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