PCR-based markers for identification of some allelic variation at Glu-1 and Glu-3 loci in common wheat

Marker assisted selection (mas) is a tool for breeding, screening, and genetic characterization ofgermplasm. allelic variation of both high and low molecular weight glutenin subunits (hmw/lmw-gs)is associated with the rheological properties of wheat flour. in this study, we investigated glutenin patternusing sds-page and their pcr based on dna markers in 60 advanced wheat lines and cultivars withdifferent origins. specific dna markers regarding to glu-1 loci, such as 1319 bp, 669 bp and 450 bpfragments were respectively validated for 2*, 17+18, 5+10 alleles. these alleles showed the highestallelic percentage in glu-1 loci in studied cultivars. however the null, 7+8 and 5+10 alleles showed thehighest allelic percentage in advanced lines. in this study, 23%, 40% and 37% of cultivars respectively,got good (10), moderate (8-9) and weak (4-7) quality scores. in advanced lines, 18%, 44% and 38% gotgood, moderate and weak quality scores respectively. ten specific dna pcr markers were also detectedfor genotyping glu-b3 alleles. the most frequent glu-b3 alleles in wheat cultivars were i, a, b and d with24%, 21%, 20% and 12%, respectively.specific pcr markers regarding to the reported glu-b3 alleleswere produced as 621bp, 1095bp, 1570 bp and 662bp consequently. the most frequent glu-b3 alleles inadvanced lines belonged to a, i and d alleles with 35%, 26% and 21% respectively. the results provideduseful information for breeding program to improve breadmaking quality and develop new cultivars