association of ssr markers with primary branches in brassica juncea l.

The study utilized f2:5 lines derived from a cross between kafiav n zagora and pusa karishma cultivars to tag genomic regions controlling primary branches in brassica juncea. one hundred and thirty f2:5 plants were used to characterize primary branch numbers, resulting in two pools of 12 genotypes for high (hpb) and low (lpb) branches. the average number of primary branches for hpb and lpb were 12.16 and 4.50, respectively. a set of 148 ssr (simple sequence repeats) markers was used for parental polymorphism screening from which 14 polymorphic ssrs were used for molecular characterization of hpb and lpb bulks, tagging genomic regions. the allelic data scored for 14 polymorphic lines was tested using student’s t-test analysis to understand relationships for primary branches with ssr markers and amplified alleles. based on this, two b-genome markers (ni2-c12 and ni2-a11) were discovered to be strongly linked to the number of primary branches. bioinformatic analysis located these two markers within a 9 mb region on chromosome b5 of b. juncea. utilising f2:5 lines of an inter-gene pool genetic cross, the current study was able to locate the loci regulating the number of primary branches on b. juncea's sub-genome chromosome b5. before proceeding with fine-mapping investigations to dissect the genomic region (between 55.9 and 64.9 mb) of sub-genome chromosome b5, it is imperative to emphasize the necessity of verifying these results across diverse genetic backgrounds.