genetic diversity of robusta coffee (coffea canephora pierre ex. a. froehner) using est-ssr markers

Research on genetic diversity demonstrated insights into variations that can be valuable for enhancing food security. expressed sequence tag (est)-simple sequence repeat (ssr) markers were employed to assess variations among thirty-nine robusta coffee accessions. in this study, the est-ssr markers utilized identified a total of 15 alleles, averaging 3.0 alleles per locus. primer cesr02 exhibited the highest polymorphic information content value at 0.59, the greatest genetic diversity value at 0.66, and the highest number of alleles (4). primers cesr04 and cesr05 revealed the highest percentage polymorphism reaching 42.86. the phylogenetic dendrogram clustered the accessions into three main groups and six subgroups. accessions 3, 26, and 30 were identified as the most genetically distinct. the most genetically related accessions represented 43.59%, while the most distinct accession recorded 2.56%. in the principal coordinate analysis (pcoa), the least genetically similar accessions constituted 20.51%, whereas 28.21% of the accessions demonstrated genetic similarity, representing the highest grouping. the findings of this study highlight the utility of est-ssr markers in identifying and categorizing coffee accessions. this approach offers valuable insights into the genetic diversity among robusta coffee accessions, facilitating further efforts in their improvement and preservation.