5-fluorouracil-loaded plga declined expression of pro-inflammatory genes il-9, il-17a, il-23 and ifn- γ in the ht-29 colon cancer cell line

Background: pro-inflammatory cytokines play critical roles in cancer pathobiology and have been considered potential targets for cancer management and therapy. understanding the impact of cancer therapeutics such as 5-fluorouracil (5-fu) on their expression might shed light on development of novel combinational therapies. this study aimed to  encapsulate 5-fu into plga  and evaluate their effects on the expression of pro-inflammatory genes il-9, il-17-a, il-23, and ifn-γ in the ht-29 cells.methods: plga-5-fu nps were constructed and characterized by dynamic light scattering (dls) and atomic force microscopy (afm). the cytotoxicity was evaluated by mtt test and, the ic50 was identified. ht-29 cells were treated with different concentrations of the plga-5-fu nps for 48 hours and, gene expression levels were analyzed by qrt-pcr. results: dls and afm analysis revealed that the prepared plga-5-fu nps were negatively charged spherical-shaped particles with a mean size of 215.9 ± 43.3 nm. plga-5-fu nps impacted the viability of ht-29 cells in a dose- and time-dependent manner. the qrt-pcr results revealed a dose-dependent decrease in the expression of il-9, il-17a, il-23 and ifn-γ genes, and their expressions were significantly different in both 10 and 20 µg/ml treated groups compared to the control. however, although the treatment of ht-29 cells with 20 µg/ml free 5-fu resulted in decreased expression of the studied genes, the differences were not statistically significant compared to the control group. conclusions: plga-5-fu nps significantly suppressed expression of the il-9, il-17a, il-23 and ifn-γ genes, and the encapsulation of 5-fu into plga improved considerably impact of the 5-fu on the ht-29 cells.