the potential association between microrna 135-5p and p62 and their effect on nrf2 pathway in multiple sclerosis

Background: multiple sclerosis (ms) is a prevalent non-traumatic disabling disease affecting young adults, characterized by complexity in its pathogenesis. nuclear factor erythroid 2-related factor 2 (nrf2) serves as a crucial transcriptional regulator of anti-inflammatory and antioxidant enzymes, influenced by the ubiquitous protein p62. it acts as a scaffold directing substrates to autophagosomes. this study aims to explore the potential association between microrna 135-5p and p62 and their impact on inflammation and oxidative stress through the nrf2 pathway in ms.methods: the study included 30 healthy controls and 60 ms patients (relapsing-remitting and secondary progressive). real-time pcr was employed for the detection of nrf2, p62, mirna135-5p, and nf-κb in serum, while p53 levels were determined using elisa.results: nrf2 and p62 expression was significantly downregulated in the ms group compared to controls. conversely, mirna135-5p, nf-κb expression, and p53 levels were significantly elevated in the ms group.conclusion: this study reveals a potential association between mirna 135-5p and p62, indicating their role in the pathogenesis of ms. results suggest that mirna 135-5p and p62 may influence inflammation and oxidative stress in ms through the nrf2 pathway, potentially mediated by nf-κb and p53.