resolvin d1 (rvd1) attenuates in vitro lps-stimulated inflammation through downregulation of mir-155, mir -146, mir -148 and krupple like factor 5

Background: chronic inflammation is associated with many inflammatory diseases. specialized pro-resolving mediators (spms) are well known for their crucial role in promoting the resolution phase of inflammation and restoring tissue homeostasis. resolvin d1 (rvd1) is an endogenous omega-3-derived lipid mediator with pro-resolving activity. this study aimed to evaluate the effect of resolvin d1 (rvd1) on some inflammatory mirnas (mir-155-5p, mir146a-5p and mir148-3p) and krüppel-like factors 5 (klf5) in an lps-stimulated thp-1 preclinical model of inflammation.methods: pma-differentiated thp-1 cells (macrophages) were pre-incubated with or without various concentrations of rvd1 (10, 50, or 100 nm) for 2 h prior to stimulation by 1 μg/ml lps. un-stimulated pma-differentiated thp-1 cells were as the control group. then, the expression levels of target genes were evaluated by real-time pcr.results: compared with untreated macrophages, stimulation with 1 µg/ml lps increased mrna expression levels of tnf-α, klf5, mir-155-5p, mir-146-5p, and mir-148a-3p. when the cells were exposed to various concentrations (10, 50 and 100 nm) of rvd1 for 2 h prior to lps stimulation, the tnf-α, klf5, mir-155-5p, mir-146-5p, and mir-148a-3p mrna expression levels were significantly downregulated in a dose-dependent manner, compared to the lps group.conclusion: the results demonstrate that rvd1 can attenuate inflammatory response in lps-stimulated macrophages. our data also showed that rvd1 may exert anti-inflammatory effects by inhibiting mir-155-5p, mir-146a-5p, and mir-148-3p.