goat polyclonal antibody against the sex determining region y to separate x- and y-chromosome bearing spermatozoa

Background: sex selection of sperm by separating x- and y-chromosome bearing spermatozoa is critical for efficiently obtaining the desired sex of animal offspring in the livestock industry. the purpose of this study was to produce a goat polyclonal antibody (pab) against the bovine sex determining region ychromosome (bsry) to separate female- and male-bearing spermatozoa.methods: to produce a goat polyclonal antibody against bsry, a female goat was subcutaneously immunized with 27 kda of recombinant bsry (rbsry) protein as the antigen. the anti-bsry pab was purified by ion-exchange chromatography. the purity of the pab was determined using the sds-pagemethod. the biological activity of the anti-bsry pab was examined using pcr to assess the binding affinity of pab for the bsry antigen and commercially sexed bull sperm.results: the total amount of purified anti-bsry pab was approximately 650 mg/goat serum (13 mg/ml). interestingly, our data showed that the binding affinity of our pab to the y bearing was high, while the binding affinity of that to the x-chromosome bearing sperm was similar to the negative control.conclusions: in conclusion, our findings show that the goat anti-sry pab specifically binds to ychromosome bearing sperm that suggesting its potential use for sex selection.