genetic regulation of interleukin-6 and interleukin-10 in covid-19 infection

Background: the role and regulation mechanisms of the interleukin-6 and 10 (il6 and il-10) serum levels and the interaction between cd4+ and cd8+ lymphocytes with sars-cov-2 igm and igg in the context of covid-19 infection are not fully understood.methods: this study was conducted on 45 covid-19 patients and 45 healthy individuals. the il-6 and il-10 promoter methylation, il-6 and il-10 gene expression, sars-cov-2 igm, and igg antibodies and cd4+ and cd8+ lymphocytes were studied by qmsp-pcr, real-time pcr, elisa, and flow cytometry techniques, respectively.results: the male ratio and mean age of critically ill patients’ group were significantly higher in compared to controls (p< 0.05). il-6 gene expression and serum levels were significantly increased in patients compared to controls (p=0.002, 0.001), but il-6 promoter methylation was not significantly decreased in patients (p=0.835). the il-10 promoter methylation and expression were not different between cases and controls (0.326, 0.455), but serum il-10 levels were higher in patients (p< 0.001). the cd4+ and cd8+ lymphocytes decreased (p< 0.001) and mean sars-cov-2 igg increased (p=0.002) in the patients compared to controls.conclusions: the covid-19 disease result in severe complications in men and elderly. the serum levels of interleukin-6 and 10 increases in covid-19 infection, and the gene expression of these two interleukins underlying in this increase. the serum levels of il-6, il-10 and sars-cov-2 igg as well as cd4+ and cd8+ lymphocyte counts should be investigated to monitor patients and predict the course of disease.