circulating levels of hotair- lncrna are associated with disease progression and clinical parameters in type 2 diabetes patients

Background: recent studies have implicated dysregulated long non-coding rna (lncrna) levels in the pathogenesis of type 2 diabetes (t2d). this study aimed to assess the expression of circulating hotair and uc.48+, examining their correlation with clinical and biochemical variables in t2d patients, pre-diabetic individuals, and healthy controls.methods: peripheral blood levels of lncrnas were quantified using qrt-pcr in 65 t2d patients, 63 pre-diabetic individuals, and 63 healthy subjects. pathway enrichment analysis was conducted to explore the functional enrichment of lncrna-mirna targets.results: analysis revealed a significantly elevated circulating level of hotair in both t2d (p < 0.0001) and pre-diabetic patients (p = 0.04) compared to controls. roc analysis demonstrated that, at a cutoff value of 9.1, with a sensitivity of 80% and specificity of 62%, hotair could distinguish t2d patients from controls (auc = 0.723, 95% ci 0.637-0.799, p < 0.0001). spearman correlation analysis identified a significant positive correlation between hotair expression, hba1c, and insulin resistance (p < 0.005). mirna enrichment analysis indicated significant enrichment of diabetes-related pathways among hotair’s mirna targets. conversely, no significant difference in uc.48+ circulating levels between groups was observed, but a significant positive correlation emerged between uc.48+ and systolic blood pressure.conclusions: this study provides evidence that elevated hotair expression levels are associated with t2d progression, suggesting their potential as biomarkers for early diagnosis and prognosis.