effect of gallic acid pretreatment and sgk1 enzyme inhibition on cardiac function and inflammation in a rat model of ischemia-reperfusion injury

Background: serum and glucocorticoid-induced kinase 1 (sgk1) is an enzyme that may play an important role in ischemic-reperfusion (i/r) injury and myocardial dysfunction. although many studies have been conducted on individual antioxidants, little attention has been paid to the effects of co-administration of an antioxidant with an sgk1 inhibitor on cardiac function after i/r.methods: this study aimed to determine the effects of gallic acid (as an antioxidant) combined with an sgk1 inhibitor on i/r-induced cardiac dysfunction and inflammation. sixty male wistar rats were randomized into 6 groups, pretreated with gallic acid or vehicle for 10 days. subsequently, the heart was isolated and exposed to i/r. in groups that received the sgk1 inhibitor, the heart was perfused with the sgk1 inhibitor gsk650394, 5 min before induction of ischemia. after that, cardiac function, inflammatory factors, and myocardial damage were evaluated.results: the combination of these two compounds improved cardiac contractility, heart rate, rate pressure product, left ventricular developed pressure, left ventricular systolic pressure, perfusion pressure, and qrs voltage significantly (p < 0.05). in addition, concomitant therapy of these two agents reduced tumor necrosis factor-alpha and interleukin-6, and the activity of creatine kinase-mb, lactate dehydrogenase, and troponin-i (p < 0.05).conclusions: the results indicated that administration of gallic acid with the sgk1 inhibitor may have a potentiating effect on the improvement of cardiac dysfunction and i/r-induced inflammation.