association of rs2954029 and rs6982502 variants with coronary artery disease by hrm technique: a gwas replication studyin an iranian population

Background: genome-wide association studies (gwas) have been the primary tool for an unbiased study of the genetic background of coronary artery disease (cad). they have identified a list of single-nucleotide polymorphisms (snps) associated with coronary artery disease (cad). in this study, we aimed to replicate the association of rs2954029 and rs6982502, a gwas identified snp, to cad in an iranian population. methods: a sample of 285 subjects undergoing coronary angiography, including 134 cad patients and 151 healthy. the genotype determination of rs2954029 and rs6982502 snps performed using the high-resolution melting analysis (hrm) technique. results: our results revealed that the tt genotype of rs2954029 (p= 0.009) and rs6982502 (p< 0.001) were significantly higher in cad patients compared with controls. binary logistic regression showed that rs6982502 and rs2954029 increase the risk of cad incidence (2.470 times, p= 0.011, 95% ci= [1.219- 4.751], and 2.174 times, p= 0.033, 95% ci= [1.066-4.433] respectively). after adjusting for confounders, we found that rs6982502 and rs2954029 are significantly associated with cad risk. conclusions: these data showed that the tt genotype of rs2954029 and rs6982502 is associated with the risk of cad in a hospital-based sample of the iranian population, which has replicated the result of recent gwas studies.