effectiveness of fe2o3 nanoparticles more than magnetic field against the destructive effect of colchicine on paramecium caudatum

Background and objective: colchicine depolymerizes microtubules and reduces their diamagnetic capacity. we investigated the effect of a static magnetic field (smf) and the fe2o3 nps on the paramecium caudatum exposed to colchicine.materials and methods: the samples were collected from temporary water sources and after identifying the species, were cultured in the laboratory. they were also sub-cultured at regular intervals (7-10 days) for purification. from pure culture, several samples were placed in the laboratory or under smf with an intensity of 61 mt for 3 days. to evaluate the effect of materials in comparison with distilled water (control), a sample (0.1 ml) of each medium was placed on a slide and exposed to 1 μl of distilled water or volume-concentrations of colchicine (0.05 to 25 μg/μl) or fe2o3 nps (0.05 to 3 μg/μl). the effect of different concentrations of fe2o3 nps in accompany with the field was also investigated. the movement of the animal was examined for 30 sec under a constant view and the sample was fixed for staining and study at the cellular level. data were analyzed using analysis of variance.results: colchicine at high concentrations (15 and 25 μg/μl) significantly reduced the motility of paramecium caudatum. magnetic field, but not nps, alone reduced cell motility. co-exposure to particles completely improved cell motility due to exposure to colchicine, however, in animals initially housed in the magnetic field, the healing effect of the particles was impaired. conclusion: the protective effects of nps may depend on the diamagnetic capacity of the microtubule.