the effects of endurance training and vitamin d supplementation on the bone resorption markers in the rats exposed to oxidative damage induced by hydrogen peroxide (h2o2)

Introduction: various studies have indicated that increased active oxygen species is associated with bone cell damage. the key role of physical exercise and vitamin d supplementation on bone health has been confirmed. the present study aimed to investigate the interactive effects of aerobic exercise with vitamin d supplementation on the bone resorption markers in the rats exposed to oxidative damage induced by hydrogen peroxide (h2o2). methods: this experimental study was conducted on 60 adult male wistar rats. the animals were randomly divided into 10 groups of six, including h2o2 (1 mmol/kg), h2o2 (2 mmol/kg), h2o2 (1 mmol/kg) with vitamin d, h2o2 (1 mmol/kg) with endurance training, h2o2 (1 mmol/kg) with vitamin d and endurance training, h2o2 (2 mmol/kg) with vitamin d, h2o2 (2 mmol/kg) with endurance training, h2o2 (2 mmol/kg) with vitamin d and endurance training, sham (dimethyl-sulfoxide with normal saline), and control. the intervention was performed for eight weeks, and the levels of tartrate-resistant acid phosphatase 5b (tracp/5b) and n-telopeptides (ntx) were measured using the elisa assay. data analysis was performed using the kolmogorov-smirnov test, two-way analysis of variance (anova), one-way anova, and tukey’s post-hoc test at the significance level of p≤0.05. results: in the animals administered with 1 and 2 mmol/kg of h2o2, no significant effects were observed on the levels of ntx (p=0.76 and p=0.47, respectively) and tracp/5b (p=0.48). on the other hand, endurance training increased the ntx levels in the rats exposed to 2 mmol/kg of h2o2 (p=0.04), while vitamin d had no significant effects on the levels of ntx (p=0.32) and tracp/5b (p=0.92). in addition, endurance training with vitamin d supplementation had no interactive effects on increased ntx and tracp/5b in the rats exposed to 1 mmol/kg (p=0.67 and p=0.99, respectively) and 2 mmol/kg of h2o2 (p=0.16 and p=0.47, respectively). conclusion: according to the results, endurance training could significantly increase the ntx level in the rats exposed to the oxidative damage induced by 2 mg/kg of h2o2.