EFFECTS OF MESENCHYMAL STEM CELLS ON TISSUE INJURY, INFLAMMATORY AND ANTI-INFLAMMATORY CYTOKINES IN CROHN’S DISEASE MODEL IN RATS

Background: mesenchymal stem cells (msc) have immune supressive effects by decreasing inflammatory cytokines (ifn-γ, tnf-α) increasing antiinflammatory cytokines (il-10) and inhibiting t cells and antigen presenting cells. various cytokines are responsible for mucosal damage in crohn‘s disease and tnf-α is one of the most important of them. the aim of this study is to determine the role of msc in the treatment of crohn‘s disease model.methods: 3% dextran sulphate sodium (dss) is used to make crohn‘s disease model. there are 7 groups which composed of 6 rats in each group; group 1: sham group, group 2: dss+ placebo, group 3: dss+ methylprednisolone 1 mg/kg, po, group 4: dss + mesalazine 100 mg/kg, po., group 5: dss+ mkh, 106 cell/rat, intraperitoneal, group 6: dss + methylprednisolone 1 mg/kg, po + msc, 10^6 cell/rat, intraperitoneal, group 7: dss + mesalazine 100 mg/kg, po + msc, 10%6 cell/rat, intraperitoneal. groups are compared with the alteration of pathological and biochemical (tnf, inf il-10) parameters. results: the average scores of group 1, 2 and 3 were higher than the average scores of group 5, 6 and 7 significantly in pathological evaluation (p<0.05). il-10 was 108.33±2.23 pg/ml in group 1, 80.83±3.62 pg/ml in group 2 where the levels of il-10 were 131.66±3.83 pg/ml, 138.33±3.19 pg/ml and 115.33±5.21 pg/ml in group 5, 6 and 7 respectively, in which msc was used as a treatment. it is observed that the average of these three groups were significantly higher (p<0.05). conclusion: this study suggest that msc may take a role in the treatment of crohn‘s disease by increasing the amount of il-10.