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fabrication of bovine serum albumin-loaded coaxial electrospun thread with an aligned core-shell fibrous structure
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نویسنده
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ghasemkhah farzaneh ,latifi masoud ,hadjizadeh afra ,shokrgozard mohammad ali
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منبع
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journal of textiles and polymers - 2020 - دوره : 8 - شماره : 1 - صفحه:75 -84
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چکیده
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Threads or yarns are key building blocks in constructing complicated fibrous structures for many biomedical applications such as scaffolds to direct cells in tissue engineering applications. in this study, proteinloaded aligned fibers with coreshell structures were successfully fabricated by combining coaxial electrospinning with a rotating disk collector. a model protein, bovine serum albumin (bsa) as a core was incorporated into shell material, including pcl/gelatin (50:50) hybrid and then electrospun aligned coreshell fibers on the rotating disk. after manually twisting the aligned fiber bundle, the prepared threads were crosslinked with genipin. the fibers and thread morphologies were characterized by scanning electron microscopy (sem), fluorescent microscopy (fm), transmission electron microscopy (tem), and fourier transform infrared spectroscopy (ftir), and the threads tensile properties were then evaluated. the wellaligned fibers (with a mean diameter of 1.472±0.444 µm) with uniform coreshell structures without any bead formation and homogeneous protein incorporation into them were obtained using a rotating wheel at 1300 rpm. bsaloaded threads with a diameter of 150250 μm were finally prepared from aligned fiber bundle by keeping their wellaligned structure. the tensile tests revealed that fibrous thread with the modulus of 34.2 cn/tex had proper tensile properties for tissue engineering approaches. ftir thus proved that the crosslinking was successful between thread and genipin. after crosslinking, threads retained their fibrous structure and demonstrated the improvement in the tensile properties (modulus increased to 40.64 cn/tex). these findings are promising to promote biomedical agentsloaded fibrous threads for tissue suturing and tissue regeneration applications.
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کلیدواژه
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coaxial electrospinning ,core-shell fiber ,aligned fibers ,fibrous thread
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آدرس
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amirkabir university of technology, nanotechnology institute, textile excellence and research center, department of textile engineering, iran, amirkabir university of technology, textile excellence and research center, department of textile engineering, iran, amirkabir university of technology, department of biomedical engineering, iran, pasteur institute of iran, national cell bank of iran, iran
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Authors
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