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molecular detection of bovine leukocytic anaplasma species in isfahan, iran
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نویسنده
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noaman vahid ,nabinejad abdolreza ,shahmoradi amirhossein ,esmaeilkhanian saeid
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منبع
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research in molecular medicine - 2016 - دوره : 4 - شماره : 2 - صفحه:47 -51
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چکیده
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Background: a. bovis and a. phagocytophilium are leukocytotropic agents of bovine anaplasmosis. they are obligate intracellular bacteria that can infect and cause anaplasmosis in human and animals. therefore, this study was carried out to detect a. bovis and a. phagocytophilum in naturally infected dairy cattle in isfahan using molecular techniques.materials and methods: in this study a total of 209 blood samples were collected from cattle in central part of iran (isfahan). the presence of a. bovis and a. phagocytophilium were examined by speciesspecific nested polymerase chain reaction (npcr) based on 16s rrna gene.results: out of the 209 cattle examined, 4 (1.99%) and 2 (1%) were found positive for a. bovis and a. phagocytophilium by npcr, respectively.conclusion: these data showed a relatively low prevalence of leukocytic anaplasma infection in cattle in central part of iran.
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کلیدواژه
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a. bovis ,a. phagocytophilium ,nested-pcr ,16s rrna gene ,iran
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آدرس
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agricultural research, education and extension organization (areeo), isfahan agriculture and natural resources research and education center, veterinary research department, ایران, agricultural research, education and extension organization (areeo), isfahan agriculture and natural resources research and education center, veterinary research department, ایران, agricultural research, education and extension organization (areeo), isfahan agriculture and natural resources research and education center, veterinary research department, ایران, agricultural research, education and extension organization (areeo), animal science research institute of iran, ایران
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Authors
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