Anti-Giardial Activity of Chloroformic Extract of Tanacetum parthenium and Artemisia annua in vitro

Background and aim: giardiasis is a one of the most prevalent intestinalparasitic diseases in human, treatment of this disease through medicinal plants isvery important since parasite resistance to chemical drugs exists. thus, in thisstudy, the in vitro anti-giardial activity of chloroformic extract of tanacetumparthenium and artemisia annua on cyst and trophozoite of giardia lamblia wereseparately investigated.methods: in this experimental study chloroformic extracts of artemisia annuaand tanacetum parthenium on cyst and trophozoite of g. lamblia in vitro wereprepared in 1, 10, 50 and 100 mg?ml concentrations for 5, 10, 30, 60 and 180 min.purified cysts were used for encystations and culture in tyi-s-33 medium. then,2 ml of each solution was placed in test tubes, to which 10,000 washed cysts andtrophozoites were added. the contents of the tubes were gently mixed andincubated. the percentages of dead parasites were determined by counting 500cysts. non treated parasites were considered a control group in each experimentand the viability of the parasites checked with eeosin staining and statisticalanalysis were done.results: the results showed that chloroformic extracts of a. annua at 100 mg/mlconcentration affected on gardia cyst 86% and 100% trophozoite after 1 hour. t.parthenium at 50mg/ml concentration killed cysts (83%) and trophozite (100%)after 1 hour, respectively. t. parthenium chloroformic extract had a better effecton cyst and trophozoite of giardia at 50 mg/ml after 1 hour exposure than a.annua extract.conclusion: according to this study, a. annua and t. parthenium chlorofomicextracts could be considered as a more effective anti-giardial agent. chloroformicextract of t. parthenium was also shown the anti-giardial activity compared witha. annua and control groups at all exposure times. therefore, in the futureresearch using these plants are recommended against giardia in low concentrationin the in vivo, also to find fractions of the pharmacological effects of these plants.