The Protective Effect of Cell Wall and Cytoplasmic Fraction of Selenium Enriched Yeast on 1, 2-Dimethylhydrazine-induced Damage in Liver

Background and aim: 1, 2-dimethylhydrazine (dmh) enhances lipidperoxidation rate by tumor mitochondria than normal tissue counterpart andcauses many disorders in antioxidant system in liver. it also increases the level ofenzymes that metabolize toxin in liver and colon. the aim of this study was toevaluate the alteration of liver and its enzymes after dmh injection and evaluateprotective effect of cell wall and cytoplasmic fractions of saccharomycescereviseae enriched with selenium (se) on these tissues.methods: forty eight female rats were prepared and acclimatized to the laboratoryconditions for two weeks, and all animals received 1, 2- dimethyl hydrazinechloride (40 mg/kg body weight) twice a week for 4 weeks except healthy control.at first colon carcinoma (aberrant crypt foci) confirmed by light microscope. thenthe changes resulting from injection of dmh on liver of animals in initial andadvanced stages of colon cancer were examined. in addition, the protective effectof cell wall and cytoplasmic fractions of selenium-enriched s. cerevisiae wereinvestigated in two phases. first phase in initial stage and second phase inadvanced stage of colon cancer were performed respectively. forty weeksfollowing the first dmh injection, all survived animals were sacrificed. then,colon and liver removed and exsanguinated by heart puncture. for measuring thelevels of enzymes (ast, alt, and alp), a commercial kit (parsazmoon, iran)and an autoanalyzer (bt 3000 pluse, italy) were used.results: the results showed that subcutaneous injection of dmh increased thealt, ast, and alp levels up to 78.5, 161.38, and 275.88 u/l compared to thecontrol, respectively. moreover, statistical analysis in both phases of experimentrevealed that the enzyme levels were decreased in the treated groups incomparison with the dmh-injected group, while the levels of these enzymes werelower in the control group.conclusion: it should be concluded that administration of cell wall andcytoplasmic fraction prepared from se-enriched s. cerevisiae could reduce thetissue damages in the livers dmh-injected rats. this beneficial effect wouldwarrant further study on the clinical application of se-enriched yeast.