Molecular identification of ovine Babesia spp. in north of Iran

background and aim: babesia is a blood-tissue parasite, which istransmitted by hard ticks from ixodidae family. the parasite is the cause ofbabesiosis among small ruminants, cattle, human, dogs and other animals. babesiais one of the main fatal factors among livestock in endemic regions such as iran.the aim of this study was to identify babesia spp infection using microscopic andmolecular methods among small ruminants in mazandaran and golestanprovinces, northern iran, during 2011-2012.methods: in this study, a total of 220 sheep and goats were selected from 22flocks in different regions of these provinces and blood samples were taken fromtheir ears. the samples were transferred to the laboratory. then thick and thinsmears were prepared, stained with geimsa and examined under light microscope.standard pcr and semi nested- pcr was performed to differentiate genus oftheileria and babesia, also identify the species of babesia.results: from a total of 220 blood samples (160 sheep and 60 goats), 34 cases(15.4 %) showed babesia infection using microscopic examination. whereas, 11cases (5%) were found positive for babesia spp using standard pcr. also, twopositive cases were showed mixed infection with theileria spp. in addition, twomicroscopic negative samples were positive by pcr assay.using semi nested-pcr, babesia ovis (n=10) and b. motasi (n=1) were detected.conclusion: our results shows ovine babesiosis is common in the northernprovinces of iran. moreover, babesia ovis is the main causative agent of ovinebabesiosis in northern iran. the relatively high prevalence of babesia infection insheep and goats indicates the epizootic stability status of babesiosis in the northernpart of iran.