design and production of sal k 1 antigens for allergy diagnosis in humans

Background: allergy is highly prevalent in different parts of the world. one of the important allergens is sal k 1 protein (from salsola kali, which is a member of the weed family). the present study aimed to design an effective sal k 1 recombinant protein for diagnostic purposes.methods: in this study, the sal k 1 gene was expressed in escherichia coli bl21(de3) cells following induction with iptg. sds-page analysis revealed a distinct protein band at 51.7 kda. protein purification was performed using a ni-nta combined molecular chromatography column under natural conditions. protein expression and diagnostic potential were subsequently confirmed through western blot analysis and enzyme linked immunosorbent assay (elisa).results: to examine the expression at different hours after induction with iptg, significant expression was observed using sds-page at 16 hours. the amount of recombinant protein in denatured conditions was more appropriate than in native conditions.conclusion: in conclusion, the successful production of recombinant sal k 1 protein may be usefulin allergy diagnostics.