the evaluation of rhoxf1 gene expression in different cell lines

Background:rhoxf1 transcription factor is highly expressed in reproductive tissues. it seems that the gene plays a role in spermatogenesis. rhoxf1 expressed in some cancerderived cell lines, too. the antiapoptotic role of rhoxf1 together with its hormonedependent function may increase its probable role in tumorigenesis as well as spermatogenesis. rhoxf1 represents the properties of cancertestis genes, which are the promising targets for immunotherapy. therefore, studying it in cancer cell lines can considerably contribute to future researches on therapeutic interventions. the aim of this study is experimental and insilico investigation of rhoxf1 gene expression in different cancer cell lines.materials and methods: in the present study, we investigated rhoxf1 insilico gene expression in the human protein atlas, gemiccl and expression atlas databases and gene expression levels in mcf7 and mdamb231 breast cancer cell lines, pc3 and du145 prostate cancer cell lines, as well as hek 293 were evaluated using rtpcr, and finally the results were compared and analyzed.results: the data obtained from the experimental study showed an increase in rhoxf1 expression levels in mcf7 cell line, which has a clear contradiction with the human atlas and gemiccl database. in this study, rhoxf1 gene expression in hek293, mdamb231, pc3 and du145 was not observed. rhoxf1 gene expression was not, likewise, in accordance with the database in a number of other cell lines, including hek 293.conclusion: we discovered the nonoccurrence of some databases together and with the experimental findings, in addition we show the increment of the expression levels of rhoxf1 gene in mcf7 breast cancer cell line, which can be examined as a possible candidate for immunotherapy.