antifungal properties of silver nanoparticles synthe‌sized from capparis spinosa fruit

Background: nanoparticles (nps) are colloidal systems with particles ranging from 10 to 100 nm in diameter. because of their large surfacevolume ratio, nps are biologically active materials that could interact with biomolecules and microorganisms, enter into the cells, and affect the metabolic functions. the study aimed to biosynthesize silver nanoparticles (agnps) from capparis spinosa fruit aqueous extract, and evaluate their ag nanostructure characterization and antifungal activity.materials and methods: capparis spinosa fruit aqueous was prepared with the percolation method. then, silver nps were synthesized using 0.01 m silver nitrate solution, and their formation was validated by color changing of the solution from green to dark brown . the nps were purified using centrifugation and then dried in an oven for further analyses. agnps nanostructure characterization was determined by various techniques such as fourier transforms infrared (ftir) spectroscopy, scanning electron microscope (sem), and ultravioletvisible (uvvis) spectroscopy. antifungal activity of agnps against three pathogenic fungi of candida albicans, candida glabrata, and kluyveromyces marxianus was also evaluated using the microdilution method.results: synthesis of agnps from aqueous extract of c. spinosa fruit was done successfully. uvvis spectrum of agnps showed an absorbance peak around 420 nm, revealing agnps surface plasmon resonance (kmax). ftir analysis showed that functional groups correspond to plant bioactive components, promoting the formation of agnps. furthermore, spherical uniformity of the synthesized agnps from plant extract was confirmed by sem analysis within the 5080 nm size range. our results showed that the produced agnps were spherical and in a suitable form and size (5080 nm). the biosynthesized agnps had an inhibitory effect against all tested fungi with the minimum inhibitory concentration of 2500, 5000, and 625 mu;g/ml and minimum bactericidal concentration of 10000, 10000, and 156.25 mu;g/ml for c. albicans, c. glabrata, and k. marxianus, respectively.conclusion: according to the uvvis spectrum, ftir, and sem results, we succeeded in synthesizing agnps from c. spinosa fruit aqueous extract. this research was the first report of agnps synthesized from aqueous extract of c. spinosa fruit. our simple, quick, and inexpensivemethod for biosynthesis of a nanoparticle, which showed antifungal activity, provides a new potential antifungal agent for therapeutic applications.