effect of ube2q on becn and cea protein in colorectal cancer cells

Background: expression of ube2q1, ube2q2, and members of the ubiquitin-conjugating enzyme family (e2) are affected in colorectal cancer (crc). the becn gene plays a key role in crc cells. in gastrointestinal carcinoma therapy, tumor-associated antigens such as cea are typically used.to investigate the association between ube2q1 and beclin1 autophagy marker and cea protein expression in ls180 crc cell line. materials and methods: in this study, changes in the expression of becn marker in ls180 cell lines with the vector containing ube2q1 were investigated using real-time pcr. the expression of cea protein was also evaluated by western blotting. statistical analyses were performed with graph pad prism software. results: the results indicated reduced expression of becn autophagy marker (p=0). therefore, in the presence of ube2q1, cancer cells have less ability to induce autophagy. however, cea protein levels in ls180 transfected cells with a ube2q1- orf-containing plasmid decreased when compared to non-transfected cells. conclusion: the use of pharmacologic inhibitors related to the autophagy mechanism can be a novel approach in cancer therapy.