aqueous cannabis extract prevents ethylene glycol-induced renal calcium crystallization

Background: some plant extracts can prevent kidney stone formation in a dose-dependent manner. in our study, we aimed to investigate the protective role of cannabis sativa aqueous extraction on calcium oxalate formation in ethylene glycolinduced rats.materials and methods: to evaluate anti-urolithiasis, variations of the main risk factors (citrate, phosphorous, calcium, and ph) have been evaluated in 24hour urine samples of rats one day before the end of the experiment. rats were divided into 4 groups: group 1 was administered regular drinking water; group 2 was administered 1% ethylene glycol in drinking water, group 3 was administered 100 mg/kg of cannabis sativa extract for oral administration by gavage along with drinking water containing 1% ethylene glycol; and group 4 was administered 200 mg/kg of cannabis sativa extract by gavage along with drinking water containing 1% ethylene glycol. finally, histopathological slides from the kidney were also evaluated. results: results showed that 100 mg/kg and 200 mg/kg doses of cannabis sativa extract significantly reduced the mentioned risk factors in comparison with ethylene glycol-treated rats (p<0.05). conclusion: histopathological slides showed reduced calcifications with extract treatment at 200 mg/kg of cannabis sativa. thus, this antioxidant herb can prevent kidney stone formation. these findings pave the way for new therapy.