evaluation of relationship between single-nucleotide polymorphism in tnf-gene promoter and susceptibility to atherosclerosis in fatemeh zahra hospital

Introduction: tumor necrosis factor alpha is a proinflammatory cytokine that initiates a polyvalent initial response of inflammatory cells, which is facilitated by coronary atherosclerosis. further, it appears that the polymorphism and susceptibility to atherosclerosis are related to the tnf-α gene promoter. aim: to assay single nucleotide polymorphisms of the tnf-α gene promoter in two sites (863 and 308) in atherosclerotic patients referred to fatemeh al-zahra hospital. materials and methods: this case-control study was conducted on 120 patients (with stenosis greater than 50%) and 120 healthy individuals (with stenosis lesser than 10%). genomic dna was extracted using the phenol-chloroform procedure from their white blood cells. genotypes of these individuals and tnf-α gene polymorphisms were analyzed by the rflp-pcr method. genotype frequencies, the hardy-weinberg equilibrium test, and chi-square analysis were conducted using the spss software version 22. results: genotype frequencies of ga, gg, and aa in position -308 of the tnf-α gene of patients were 12.5%, 75%, and 12.5%, respectively; in healthy subjects, they were 7.5%, 21.7%, and 70.8%, respectively. allele a to g allele increased the risk of disease by 12.716%. the genotype frequencies of ac, cc, and aa in position -863 of the tnf-α gene of patients were 3.3%, 69.2%, and 27.5%, respectively; in healthy individuals, they were 2.5%, 11.7%, and 85.8%, respectively. allele a to c allele increased the risk of disease by 16.373%. statistical analysis revealed a significant correlation between the risk of atherosclerosis with single nucleotide polymorphisms in the tnf-α gene-863 at c < a and in gene -308 at g < a (p < 0.05). conclusion: according to this study, mutations in the promoter region of the tnf-α gene possibly increased susceptibility to atherosclerosis. therefore, genotype determination of individuals in these areas can help to identify patients with high risk for cardiac disease.