مقایسه اثرسنجی محلول هوواسان تی آر 50 (huwa-san tr-50) با سبز مالاشیت و فرمالین در کنترل آلودگی قارچی تخم ماهی قزل آلای رنگین کمان در مراحل مختلف تفریخ

ﻫﻮاﺳﺎن ﺗﯽ آر 50 ﯾﮏ ﺿﺪ ﻋﻔﻮﻧﯽ ﮐﻨﻨﺪه ﺑﺮ ﭘﺎﯾﻪ ﭘﺮاﮐﺴﯿﺪ ﻫﯿﺪروژن ﻣﯽ ﺑﺎﺷﺪ . ﻫﺮ ﻟﯿﺘﺮ از اﯾﻦ ﺗﺮﮐﯿﺐ ﺣﺎوی 570 ﮔﺮم ﭘﺮاﮐﺴﯿﺪ ﻫﯿﺪروژن ﺑﻮده وﻧﻈﺮ ﺑﻪ ﻧﺎﭘﺎﯾﺪاری اﯾﻦ ﻣﺤﻠﻮل ، ﯾﻮن ﻧﻘﺮه ﺑﻪ ﻋﻨﻮان ﺗﺜﺒﯿﺖ ﮐﻨﻨﺪه ﺑﻪ ﻣﯿﺰان 0/36 ﮔﺮم ﺑﻪ آن اﺿﺎﻓﻪ ﮔﺮدﯾﺪه اﺳﺖ . ﻫﺪف از اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﺮرﺳﯽ ﺗﻌﯿﯿﻦ ﮐﺎراﯾﯽ اﯾﻦ ﻣﺤﻠﻮل در ﻣﻬﺎر و ﮐﺎﻫﺶ ﺗﻠﻔﺎت ﻧﺎﺷﯽ از آﻟﻮدﮔﯽ ﻗﺎرﭼﯽ در ﻫﭽﺮی ﻣﺎﻫﯿﺎن ﻗﺰل اﻻی رﻧﮕﯿﻦ ﮐﻤﺎن ﻃﯽ دوره اﻧﮑﻮﺑﺎﺳﯿﻮن، در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﺳﺒﺰ ﻣﺎﻻﺷﯿﺖ و ﻓﺮﻣﺎﻟﯿﻦ ﺑﻮد. ﺑﺮای اﻧﺠﺎم اﯾﻦ ﮐﺎر از 27 ﺗﺮاف ﮐﺎﻟﯿﻔﺮﻧﯿﺎﯾﯽ در 9 ﺗﯿﻤﺎر و ﺳﻪ ﺗﮑﺮار اﺳﺘﻔﺎده ﮔﺮدﯾﺪ و در ﻫﺮ ﺗﺮاف ﺗﻌﺪاد 700 ﻋﺪد ﺗﺨﻢ ﻟﻘﺎح ﯾﺎﻓﺘﻪ ﻣﺎﻫﯽ ﻗﺰل آﻻی رﻧﮕﯿﻦ ﮐﻤﺎن ذﺧﯿﺮه ﺳﺎزی ﮔﺮدﯾﺪ. . در اﯾﻦ ﺑﺮرﺳﯽ 5 ﺗﯿﻤﺎر ﺑﺎ ﭘﻨﺞ دوز ﻣﺨﺘﻠﻒ ﻫﻮآﺳﺎن ﺗﯽ آر 50 (300 ،200 ،100 ،50 و 500 ﭘﯽ ﭘﯽ ام) ، ﯾﮏ ﺗﯿﻤﺎر ﺑﺎ ﻣﺎﻻﺷﯿﺖ ﮔﺮﯾﻦ ﺑﺎ دوز 2 ﭘﯽ ﭘﯽ ام و ﯾﮏ ﺗﯿﻤﺎر ﺑﺎ ﻓﺮﻣﺎﻟﯿﻦ ﺑﺎ دوز 1000ﭘﯽ ﭘﯽ ام، ﮐﻪ ﺑﻪ ﻣﺪت 30 دﻗﯿﻘﻪ از ﻃﺮﯾﻖ ﺣﻤﺎم دادن ﺿﺪ ﻋﻔﻮﻧﯽ ﺷﺪﻧﺪ؛ ﯾﮏ ﺗﯿﻤﺎر ﺷﺎﻫﺪ ﻣﺜﺒﺖ )آﻟﻮده ﺑﻪ ﻗﺎرچ و ﺑﺪون درﻣﺎن داروﯾﯽ( و ﯾﮏ ﺗﯿﻤﺎر ﺷﺎﻫﺪ ﻣﻨﻔﯽ )ﻏﯿﺮ آﻟﻮده ﺑﻪ ﻗﺎرچ و ﺑﺪون درﻣﺎن داروﯾﯽ( ﻧﯿﺰ در ﻧﻈﺮ ﮔﺮﻓﺘﻪ ﺷﺪ. در ﻃﻮل دوره اﻧﮑﻮﺑﺎﺳﯿﻮن ﺷﺮاﯾﻂ ﺑﺮای ﺗﻤﺎﻣﯽ ﺗﯿﻤﺎرﻫﺎ ﯾﮑﺴﺎن ﺑﻮد ﻃﯽ اﯾﻦ ﺑﺮرﺳﯽ ﻣﺸﺨﺺ ﺷﺪ . ﮐﻤﺘﺮﯾﻦ ﻣﯿﺰان ﻗﺎرچ زدﮔﯽ ﺗﺨﻢ ﻫﺎ در دوز 300 ﭘﯽ ﭘﯽ ام ﻫﻮاﺳﺎن ﺗﯽ ار 50 ﻣﺸﺎﻫﺪه ﮔﺮدﯾﺪ ﮐﻪ ﺑﺎ ﺗﯿﻤﺎرﻫﺎی ﻣﺎﻻﺷﯿﺖ و ﻓﺮﻣﺎﻟﯿﻦ ، اﺧﺘﻼف ﻣﻌﻨﯽ داری از ﻧﻈﺮ آﻣﺎری ﻧﺪاﺷﺖ ﻫﻤﭽﻨﯿﻦ ﺑﺎﻻﺗﺮﯾﻦ درﺻﺪ ﺑﺎزﻣﺎﻧﺪﮔﯽ ﺗﺨﻢ ﻫﺎ ﺗﺎ ﻣﺮﺣﻠﻪ ﭼﺸﻢ زدﮔﯽ ﻧﯿﺰ ﻣﺮﺑﻮط ﺑﻪ ﺗﯿﻤﺎر 300 ﭘﯽ ﭘﯽ ام ﺑﻮد ﮐﻪ ﺑﺎ ﺗﻤﺎم ﺗﯿﻤﺎرﻫﺎ اﺧﺘﻼف ﻣﻌﻨﯽ دار ﻧﺸﺎن داد. ﺑﺮ اﺳﺎس ﻧﺘﺎﯾﺞ ﺑﺪﺳﺖ آﻣﺪه دوز 300 ﭘﯽ ﭘﯽ ام ﻫﻮواﺳﺎن مناﺳﺐ ﺗﺮﯾﻦ ﻏﻠﻈﺖ ﺑﺮای ﺿﺪ ﻋﻔﻮﻧﯽ ﮐﺮدن ﺗﺨﻢ ﻫﺎ اﺳﺖ

comparison of huwa-san tr-50 solution efficiency with malachite green and formalin on fungal contamination control of rainbow trout (oncorhynchus mykiss) eggs in incubation stage

this research was conducted in autumn and winter of 2016 in shahid motahary coldwater fishes genetic and breeding research center. the purpose of this study was to evaluate the effect of huwa-san tr-50 solution on controlling rainbow trout fungus contamination at incubation stage and comparing its effects with malachite green and formalin. to do this, 27 californian traphs were used in 9 treatments and 3 replicates, and 700 eggs of rainbow trout were stoked  in each traph. 5 treatments with different concentrations (50, 100, 200, 300 and 500 ppm) of huwa-san tr-50 solution, one treatment with malachite green (2ppm) and one treatment with formalin (1000ppm), by bathing for 30 minutes were disinfected; a positive control (contaminated with fungi and without medication) and a negative control (non-infected with fungi and without medication) were also considered. the results showed that solution of 300 ppm huwa-san tr-50 was suitable concentration for disinfection of eggs. percentage of eyed egg and hatching at higher doses and lower than 300 ppm huwa-san tr-50 showed a decreasing trend.