aflatoxin m1 determination in ice cream based on immunoaffinity column clean-up followed by hplc-fld

Background: aflatoxin m1 (afm1) in industrial ice cream was analyzed by immunoaffinity column (iac) clean-up followed by high-performance liquid chromatography (hplc) with a fluorescence detector (fld) as a highly-sensitive method to confirm and quantify afm1 in the ice cream samples.methods: a total of 150 industrial ice cream samples were randomly collected from supermarkets in seven iranian cities (tehran, tabriz, karaj, urmia, sanandaj, qazvin, and khoramabad).results: afm1 was detected in more than 80.0% of samples. the average afm1 concentration in ice cream was 29.79 ng/l. according to the results, in 12.62% of samples, afm1 levels exceeded the maximum european union (eu) limits for adults (50 ng/ml). this study recommends regular monitoring of afm1 concentration in ice cream in iran.conclusion: results of this study indicate the need to develop a database to assist law enforcement agencies, traders, and policymakers in local governments to minimize or prevent health-related risks caused by afm1.