ارزیابی اثرات ضد‌میکروبی و آنتی اکسیدانی عصاره جلبک‌های padina وsargassum بومی خلیج عمان در مقابل باکتری‌های ویبریو عامل بیماری نکروز حاد هپاتوپانکراس میگو

ﻫﺪف ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ ارزﯾﺎﺑﯽ ﻓﻌﺎﻟﯿﺖﻫﺎی ﺿﺪﻣﯿﮑﺮوﺑﯽ و آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ﺟﻠﺒﮏﻫﺎی . padina spو .sp sargassum ﻧﻤﻮﻧﻪ ﺑﺮداری ﺷﺪه از ﺧﻠﯿﺞ ﻋﻤﺎن ﺑﻮد. ﺑﺪﯾﻦ ﻣﻨﻈﻮر در ﺑﻬﺎر 1403ﻧﻤﻮﻧﻪ ﺑﺮداری از ﺟﻠﺒﮏﻫﺎی padinaو sargassum در ﺳﻮاﺣﻞ ﺷﻬﺮﺳﺘﺎن ﺟﺎﺳﮏ اﻧﺠﺎم ﺷﺪ. ﻋﺼﺎرهﮔﯿﺮی از ﻧﻤﻮﻧﻪﻫﺎی ﺟﻠﺒﮏ ﺗﻮﺳﻂ ﺗﺮﮐﯿﺐ روشﻫﺎی ﺧﯿﺴﺎﻧﺪن و ﺣﻤﺎم اوﻟﺘﺮاﺳﻮﻧﯿﮏ اﻧﺠﺎم ﺷﺪ. ﺳﭙﺲ ﻣﯿﺰان ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ﻋﺼﺎرهﻫﺎ ﺑﻪ روش ﻣﻬﺎر رادﯾﮑﺎلﻫﺎی آزاد دی ﻓﻨﯿﻞ1- ﭘﯿﮑﺮﯾﻞ ﻫﯿﺪرازﯾﻞ اﻧﺠﺎم ﺷﺪ. ﺗﻌﯿﯿﻦ ﮐﻤﺘﺮﯾﻦ ﻏﻠﻈﺖ ﺑﺎزدارﻧﺪﮔﯽ (mic) و ﮐﺸﻨﺪﮔﯽ ﺑﺎﮐﺘﺮی (mbc) ﺑﻪ روش ﻣﺎﯾﮑﺮوداﯾﻠﻮﺷﻦ ﺑﺮاث ارزﯾﺎﺑﯽ ﺷﺪ. ﻧﺘﺎﯾﺞ ﻣﯿﺎﻧﻪ ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ﻋﺼﺎرهﻫﺎی آﺑﯽ و آﻟﯽ ﺟﻠﺒﮏ padina ﺑﺘﺮﺗﯿﺐ ﻣﻌﺎدل µg/ml 80/6±93/64 و 474/41±3/53µg/ml و sargassum ﻣﻌﺎدلµg/ml 65/4±87/11 و µg/ml 325/26±2/23 ﺑﻮد. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد mic ﻋﺼﺎره آﻟﯽ padina در ﻣﻘﺎﺑﻞ v. alginolyticus ، v. parahaemolyticus و v. harveyi ﺑﺘﺮﺗﯿﺐ ﻣﻌﺎدلµg/ml 256، 64 و 64 ﺛﺒﺖ ﺷﺪ. mbc آنﻫﺎ ﺑﺘﺮﺗﯿﺐ ﻣﻌﺎدلµg/ml 256 ،512 و 128 ﺛﺒﺖ ﺷﺪ. در ﺣﺎﻟﯿﮑﻪ ﻣﯿﺰان mic ﻋﺼﺎره آﺑﯽ padina در ﻣﻘﺎﺑﻞ اﯾﻦ ﺑﺎﮐﺘﺮیﻫﺎ در ﻏﻠﻈﺖﻫﺎی 512، 256 و 128 و mbc آن ﺑﺘﺮﺗﯿﺐ ﺑﺪون ﻓﻌﺎﻟﯿﺖ،µg/ml 512 و 256 ﺛﺒﺖ ﺷﺪ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد mic ﻋﺼﺎره آﻟﯽ sargassum در ﻣﻘﺎﺑﻞ ﺑﺎﮐﺘﺮیﻫﺎی ﻣﺬﮐﻮر ﺑﺘﺮﺗﯿﺐ µg/ ml 128، 64 و 128 و mbc آن ﻣﻌﺎدلµg/ml 256، 128، 128 ﺛﺒﺖ ﺷﺪ. ﻣﯿﺰان mic ﻋﺼﺎره آﺑﯽ sargassum ﻧﯿﺰ در ﻣﻘﺎﺑﻞ اﯾﻦ ﺑﺎﮐﺘﺮﯾﻬﺎ ﻣﻌﺎدلµg/ ml 256، 256و 128ﺗﻌﯿﯿﻦ ﺷﺪ. اﯾﻦ ﻧﺘﺎﯾﺞ ﻓﻌﺎﻟﯿﺖ ﻋﺼﺎره ﺟﻠﺒﮑﯽ را در ﻣﻬﺎر رﺷﺪ ﺑﺎﮐﺘﺮیﻫﺎی وﯾﺒﺮﯾﻮ ﺛﺎﺑﺖ ﮐﺮد. از اﯾﻨﺮو ﺟﻠﺒﮏﻫﺎی ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﻣﯽ ﺗﻮاﻧﻨﺪ در ﺟﯿﺮه ﻏﺬاﯾﯽ آﺑﺰﯾﺎن ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﺗﮑﻤﯿﻠﯽ ﻗﺮار ﮔﯿﺮﻧﺪ.

evaluation of the antimicrobial and antioxidant effects of padina and sargassum algae extracts against vibrio species causing acute hepatopancreatic necrosis disease in shrimp

the present study aimed to evaluate the antimicrobial and antioxidant activities of padina sp. and sargassum sp. algae collected from the gulf of oman. samples were obtained from the coast of jask city in spring 2024. algal extracts were prepared using a combination of soaking and ultrasonic bath methods. antioxidant activity was assessed using the diphenyl-1-picrylhydrazyl (dpph) free radical scavenging assay, while antimicrobial activity was determined by broth microdilution to measure the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc). the median antioxidant activities of aqueous and organic extracts were 80.93 ± 6.64 and 474.3 ± 41.53 µg/ml for padina, and 65.87 ± 4.11 and 325.2 ± 2.26 µg/ml for sargassum, respectively. the mics of padina organic extracts against vibrio parahaemolyticus, v. alginolyticus, and v. harveyi were 256, 64, and 64 µg/ml, with mbcs of 512, 256, and 128 µg/ml, respectively. aqueous extracts of padina were less effective, with mics of 512, 256, and 128 µg/ml and mbcs of 512 and 256 µg/ml. for sargassum, the organic extract exhibited stronger antimicrobial activity, with mics of 128, 64, and 128 µg/ml and mbcs of 256, 128, and 128 µg/ml, respectively. the mics of aqueous sargassum extracts were 256, 256, and 128 µg/ml. overall, these findings confirm the antioxidant and antibacterial potential of padina and sargassum extracts, particularly their activity against pathogenic vibrio species. these algae therefore represent promising candidates for development as dietary supplements in aquaculture