age-specific reference intervals for routine laboratory biochemical tests in the iranian adolescent girls

Introduction: for interpretation of the most common biochemical markers reference intervals (ris) are widely considered and reported. therefore, in this study, we aimed to determine age-specific ris for some biochemical tests by removing and/or limiting critical gaps including sex, age group, geographical region, measurement device, and type of kits and reagents for routine laboratory biochemical variables in healthy adolescent girls aged 12-19 years. materials and methods: in this cross-sectional study a total of 1026 adolescent girl was recruited. according to the study aim, the levels of serum for some routine biochemical tests were measured on a bt-3000 auto-analyzer. on the basis of the non-parametric rank method and by use of clsi ep28-a3 guidelines, we determined age-specific ris, including confidence interval 90%, under specific exclusion criteria. results: age partitioning was required for none of the upper and lower limits of biochemical markers. all serum biochemical tests concentration remained relatively constant throughout the age range. conclusion: by removing and/or limiting critical gaps involved in reference values of biochemical tests, it has provided an important understanding of metabolic processes and it has facilitated clinical application to track different diseases better.