interactive of arsenate and phosphate on arsenic-induced oxidative stress in root of isatis cappadocica desv.

In present study, growth, arsenic accumulation, and antioxidant responses in root of isatis cappadocica were investigated in response to application of arsenate and phosphate. reduction in root dry weights was significant with 1200 micro;m arsenate and 5 micro;m phosphate treatments. phosphate had a steady effect on root dry weight improvement, especially in high arsenate treatments ge; 200 mu;m. as concentrations in roots increased with increasing arsenate supply levels, and decreased markedly with increasing phosphate in each arsenate treatment.. applied arsenate induced oxidative stress, which caused increasing the concentrations of hydrogen peroxide (h2o2) and malondialdehyde (mda). the activities of antioxidant enzyme such as catalase (cat), peroxidase (pod) and ascorbate peroxidase (apx) in isatis cappadocica roots increased significantly from 200800 micro;m arsenate and 5 micro;m phosphate treatments while their activities were decreased at 1200 micro;m arsenate and 5 micro;m phosphate, compared with control plants. the phosphate mediated decline of cat, pod and apx activities, which could be an important factor in regulating asinduced oxidative stress in roots. our results concluded that, i. cappadocica has a great capability to tolerance and accumulate the as . also, application of phosphate has a clearly vital role in oxidative stress reduction in roots of exposed i. cappadocica to arsenate.