Investigation of Polymorphisms of ACEII Gene in People With Coronavirus With Severe and Mild Symptoms or Asymptomatic

Introduction: angiotensin-converting enzyme 2 (ace2) is the central receptor of coronavirus disease 2019 (covid-19) in host cells. genetic polymorphisms in the ace2 gene may promote cardiovascular disease and systemic inflammatory injury in a patient affected by covid-19. thus, the genetic background may account for the substantial inter-individual diversity in illness susceptibility or severity. our study was conducted to find a significant relationship between ace2 rs4646142 and rs2285666 polymorphisms and susceptibility to severe acute respiratory syndrome coronavirus 2 (sars‑cov‑2). methods: in this study, we randomly selected 230 samples, including 76 patients with severe clinical symptoms and 154 patients with mild clinical symptoms (the positive case of covid-19 was confirmed by real-time reverse transcriptase polymerase chain reaction [rt-pcr] assay). then, we performed dna extraction and investigated the polymorphisms of rs2285666 and rs4646142 by rflp-pcr method with taqi and alu1 restriction enzymes. results: the study population included 107 men and 123 women, and the mean (±sd) age of the participants was 42.66±10.2. first, the levels of igm and igg were examined, and a significant association was observed in the level of igm between the two groups of covid-19 patients with mild and severe symptoms, as opposed to igg. meanwhile, no significant difference was observed between ace2 rs4646142 and rs2285666 polymorphisms and the severity of covid-19. conclusion: to better understand the genetic variations in people’s susceptibility to covid-19, this study was designed to evaluate the association between various ace2 polymorphisms and the infection risk of sars-cov-2. however, no statistical difference was discovered.