Effects of Pyrroloquinoline Quinone (PQQ) on Ischemia-Reperfusion Injury in Rat Ovaries: Histological and Biochemical Assessments

Objective: to evaluate effects of pyrroloquinoline quinone (pqq) on ischemia-reperfusion injury using a rat ovary model. method: thirty healthy female wistar rats with 250g were randomized into five experimental groups (n=6): group sham: the rats underwent only laparotomy. group ischemia: a 3- hour ischemia only. group i/r: a 3-hour ischemia and a 3-hour reperfusion. 30 min before termination of reperfusion 20 μl soybeen oil (solvent of pqq) was administered. group i/pqq: a 3-hour ischemia only and 20 μl (10 mg/kg) intraperitoneal administration (ip) of pqq 2.5 hours after induction of ischemia. group i/r/pqq: a 3-hour ischemia, a 3-hour reperfusion and 20 μl (10 mg/kg) ip of pqq 2.5 hours after induction of ischemia. results: animals treated with pqq showed significantly ameliorated development of ischemia and reperfusion tissue injury compared to those of other groups (p=0.001). the significant higher values of sod, gpo and gst were observed in i/r/pqq animals compared to those of other groups (p=0.001). damage indicator (mda) was significantly lower in i/r/pqq animal compared to those of other groups (p=0.001). conclusion: intraperitoneal administration of pqq could be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia.