Interaperitoneal Administration of Αlpha-Tocopherol Loaded Nanoparticles Improves Ischemia-Reperfusion Injury in Rat Ovaries Torsion and Detorsion Model

Objective: to investigate effects of intraperitoneally administration of α-tocopherol loaded nanoparticles (tnp) on ischemia-reperfusion injury in ovaries. methods: thirty-five healthy female wistar rats ~250g were randomized into seven experimental groups (n=5): group sham: the rats underwent only laparotomy. group ischemia: a 3- hour ischemia only. group i/r: a 3-hour ischemia and a 3-hour reperfusion. group i/t: a 3-hour ischemia only and 100 mg/kg intraperitoneal administration (ip) of α-tocopherol 2.5 hours after induction of ischemia. group i/r/t: a 3-hour ischemia, a 3-hour reperfusion and 100 mg/kg ip of α-tocopherol 2.5 hours after induction of ischemia. group i/tnp: a 3-hour ischemia only and 1 mg/kg ip of tnp 2.5 hours after induction of ischemia. group i/r/tnp: a 3-hour ischemia, a 3-hour reperfusion and 1 mg/kg ip of tnp 2.5 hours after induction of ischemia. results: animals treated with αtnp showed significantly ameliorated development of ischemia and reperfusion tissue injury compared to those of other groups (p=0.001). the significant higher values of sod, tgsh, gpo, gshrd and gst were observed in i/r/nc animals compared to those of other groups (p=0.001). damage indicators (nos, mda, mpo and dna damage level) were significantly lower in i/r/nc animal compared to those of other groups (p=0.001). conclusion: intraperitoneal administration of tnp could be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia.