Biosynthesis of Copper and Iron Nanoparticles using Neem (Azadirachta indica) Leaf Extract and their Anti-bacterial Activity

Neem leaves extract was use to synthesize metal (iron and copper) nanoparticles. the nanoparticles were characterized using scanning electron microscope (sem), fourier transform infrared (ftir) and uv-vis spectrometer. the formation and stability of the reduced metal nanoparticles in the colloidal solution were monitored by uv–vis spectrophotometer analysis. their peaks of cunps and fenps were determined with a particles size in the range of 250nm-550nm and 250nm-350nm and have shapes of spherical and dendrimer respectively. the zone of inhibition by cunp on gram negative bacteria (staphylococcus aureus) is 20mm ± 1 while on gram positive bacteria (escherichia coli) is 12.5mm ± 1. thus, the synthesized metal nanoparticles prove to have very high anti- bacterial activity on both staphylococci aureus and escherichia coli. copper nanoparticles exhibits high antibacterial activity compared to iron nanoparticles.