Optimization and interactive effects of salinity,fertilizer and inoculums concentration on biodegradation in marine environment

With the speeding up of the exploitation and shipments of petroleum,the oil spill or off-shore oil production all over the world have dramatically increased. bioremediation has been recognized as a cost-effective and environment-friendly approach for cleanup of the petroleum pollutants from marine environment. though some studies have reported the effect of every factor on the biodegradation,there are few reports on the interactive and cumulative effect of environment factors on biodegradation. in this paper,interactive effects of salinity,fertilizer and inoculums concentrations on biodegradation were studied by response surface methodology,in order to provide a better theoretical basis for using biodegradation in the marine environment. the results indicated that there was good agreement between the predicted values and experimental values. further analyzing the variance (anova),the model f-value indicated the model was significant for biodegradation,and a second-order polynomial equation was consequently defined. additionally,predicted value vs. actual value and residual value vs. predicted value were also analyzed and the results have shown that the model was effective. lastly,according to the interactive effects of three factors,the factors were ranked by descending order: salinity>inoculums concentration ≈ nitrogen and phosphorus ratio. © 2017,university of kuwait. all rights reserved.