تاثیر 4 هفته تمرین هوازی بر عملکرد شناختی و پویایی میتوکندریایی در بافت هیپوکمپ رت‌های نر ویستار مبتلا به بیماری آلزایمر

ﻫﺪف از ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ، ﺑﺮرﺳﯽ اﺛﺮ 4 ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ ﻫﻮازی ﺑﺮ ﻋﻤﻠﮑﺮد ﺷﻨﺎﺧﺘﯽ و ﭘﻮﯾﺎﯾﯽ ﻣﯿﺘﻮﮐﻨﺪرﯾﺎﯾﯽ در ﺑﺎﻓﺖ ﻫﯿﭙﻮﮐﻤﭗ رتﻫﺎی ﻧﺮ وﯾﺴﺘﺎر ﻣﺒﺘﻼ ﺑﻪ ﺑﯿﻤﺎری آﻟﺰاﯾﻤﺮ ﺑﻮد. روش ﮐﺎر: ﺑﺪﯾﻦ ﻣﻨﻈﻮر، ﺗﻌﺪاد 21 ﺳﺮ رت ﻧﺮ ﺑﺎﻟﻎ ﻧﮋاد وﯾﺴﺘﺎر 20 ﻣﺎﻫﻪ ﺑﻪ روش ﺗﺼﺎدﻓﯽ ﺳﺎده ﺑﻪ 3 ﮔﺮوه ﺑﯿﻤﺎری آﻟﺰاﯾﻤﺮ ﺗﻌﺪاد = 7 ﺳﺮ ، ﮔﺮوه ﺑﯿﻤﺎری آﻟﺰاﯾﻤﺮ + ورزش ﻫﻮازی ﺗﻌﺪاد = 7 ﺳﺮ و ﮔﺮوه ﮐﻨﺘﺮل ﺗﻌﺪاد = 7 ﺳﺮ ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. اﻟﻘﺎی ﺑﯿﻤﺎری آﻟﺰاﯾﻤﺮ از ﻃﺮﯾﻖ ﺗﺰرﯾﻖ داﺧﻞ ﻫﯿﭙﻮﮐﻤﭙﯽ aβ42 aβ ﻫﺮ ﻃﺮف 1 ﻣﯿﮑﺮوﻟﯿﺘﺮ ﺻﻮرت ﭘﺬﯾﺮﻓﺖ. ﻫﻔﺖ روز ﺑﻌﺪ از ﺟﺮاﺣﯽ، ﮔﺮوه ورزش 4 ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ دوﯾﺪن روی ﻧﻮارﮔﺮدان 5 روز در ﻫﻔﺘﻪ ﺑﺎ ﺳﺮﻋﺖ 10 اﻟﯽ 15 ﻣﺘﺮ ﺑﺮ دﻗﯿﻘﻪ را اﺟﺮا ﻧﻤﻮدﻧﺪ. ﭼﻬﻞ و ﻫﺸﺖ ﺳﺎﻋﺖ ﭘﺲ از آﺧﺮﯾﻦ ﺟﻠﺴﻪ ﺗﻤﺮﯾﻨﯽ ﺣﯿﻮاﻧﺎت ﺗﺤﺖ آزﻣﻮن رﻓﺘﺎری ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﺑﯿﺴﺖ و ﭼﻬﺎر ﺳﺎﻋﺖ ﺑﻌﺪ از آزﻣﻮن رﻓﺘﺎری ﺗﻤﺎﻣﯽ رتﻫﺎ ﮐﺸﺘﻪ ﺷﺪﻧﺪ و ﺑﺎﻓﺖ ﻫﯿﭙﻮﮐﻤﭗ اﺳﺘﺨﺮاج ﺷﺪ. ﺑﯿﺎن mrna ژنﻫﺎی mfn2 ،opa1 و drp1 ﺑﺎ اﺳﺘﻔﺎده از روش realtime pcr ﻣﻮرد ﺳﻨﺠﺶ ﻗﺮار ﮔﺮﻓﺖ. ﺑﺮای ﺗﺠﺰﯾﻪ و ﺗﺤﻠﯿﻞ آﻣﺎری از آزﻣﻮن ﺗﺤﻠﯿﻞ وارﯾﺎﻧﺲ ﯾﮏ ﻃﺮﻓﻪ اﺳﺘﻔﺎده ﺷﺪ. ﯾﺎﻓﺘﻪﻫﺎ: ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﻋﻤﻠﮑﺮد ﯾﺎدﮔﯿﺮی و ﺣﺎﻓﻈﻪ ﻓﻀﺎﯾﯽ و ﻫﻤﭽﻨﯿﻦ ﻣﯿﺰان ﺑﯿﺎن ژنﻫﺎی opa1 و mfn2 در ﺣﯿﻮاﻧﺎت ﮔﺮوه ﺑﯿﻤﺎری آﻟﺰاﯾﻤﺮ در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﮔﺮوه ﮐﻨﺘﺮل ﮐﺎﻫﺶ ﯾﺎﻓﺖ 0/05

the effect of 4 weeks of aerobic exercise on cognitive function and mitochondrial dynamics in hippocampal tissue of male wistar rats with alzheimer's disease

aim: the aim of the present study was to investigate the effect of 4 weeks of aerobic exercise on cognitive function and mitochondrial dynamics in the hippocampal tissue of male wistar rats with alzheimer's disease.method: for this purpose, 21 male wistar rats at 20 months of age were randomly divided into 3 groups: alzheimer's disease n=7 , alzheimer's disease+aerobic exercise n=7 and control group n=7 . alzheimer's disease was induced by intra hippocampal injection of aβ42 1 microliter per side . seven days after surgery, the exercise group performed 4 weeks of treadmill training 5 days per week at a speed of 10 to 15 m/min . forty eight hours after the last training session, the animals underwent behavioral tests. twenty four hours after the behavioral test, all rats were killed and hippocampal tissue was extracted. the mrna expression of opa1, mfn2 and drp1 genes was assayed using real time pcr. one way analysis of variance was used for statistical analysis.results: the results showed that spatial learning and memory performance as well as the gene expression of opa1 and mfn2 in animals with alzheimer's disease decreased compared to the control group p<0.05 , while the gene expression of drp1 increased p<0.05 . aerobic exercise in patient animals improved spatial learning and memory performance p<0.05 , increased hippocampal opa1 and mfn2 genes expression p<0.05 , and decreased drp1 gene expression compared with alzheimer's disease group p<0.05 .conclusion: in general, it seems that aerobic exercise can improve spatial learning and memory performance in alzheimer's disease by modulating abnormal mitochondrial dynamics.