پاسخ حاد میلوپراکسیداز و وضعیت تام اکسیدانی به فعالیت ورزشی فزاینده در مردان میانسال تمرین کرده و بدون تمرین

ﻫﺪف: ﻧﻮﺗﺮوﻓﯿﻠﻬﺎ ﺣﺎوی آﻧﺰﯾﻢ ﻣﯿﻠﻮﭘﺮاﮐﺴﯿﺪاز ﻫﺴﺘﻨﺪ ﮐﻪ ﺑﺎ ﺗﻮﻟﯿﺪ اﮐﺴﯿﺪاﻧﻬﺎ و ﮐﺎﺗﺎﻟﯿﺰ اﺳﯿﺪ ﻫﺎﯾﭙﻮﮐﻠﺮوس در ﺑﺴﯿﺎری از ﻓﺮاﯾﻨﺪ ﻫﺎی ﺳﻠﻮﻟﯽ ﻧﻘﺶ دارﻧﺪ. از ﻃﺮﻓﯽ، از ﺳﻨﯿﻦ ﻣﯿﺎﻧﺴﺎﻟﯽ ﻣﺤﺎﻓﻈﺖ ﻓﯿﺰﯾﻮﻟﻮژﯾﮑﯽ ﺑﺪن در ﺑﺮاﺑﺮ اﺳﺘﺮس اﮐﺴﯿﺪاﺗﯿﻮ ﺷﺮوع ﺑﻪ ﮐﺎﺳﺘﻦ ﻣﯽ ﮐﻨﺪ ﺑﻨﺎﺑﺮاﯾﻦ ﻫﺪف ﺗﺤﻘﯿﻖ ﺣﺎﺿﺮ ﻣﻄﺎﻟﻌﻪ ﺗﻐﯿﯿﺮات ﻣﯿﻠﻮﭘﺮاﮐﺴﯿﺪاز و وﺿﻌﯿﺖ ﺗﺎم اﮐﺴﯿﺪاﻧﯽ در ﻣﺮدان ﻣﯿﺎﻧﺴﺎل ﺳﺎﻟﻢ ﺷﺮﮐﺖ ﮐﻨﻨﺪه در ﻓﻌﺎﻟﯿﺘﻬﺎی ﻣﻨﻈﻢ ورزش ﻫﻤﮕﺎﻧﯽ و اﻓﺮاد ﺑﺪون ﺗﻤﺮﯾﻦ اﺳﺖ. روش ﮐﺎر: ﺑﯿﺴﺖ ﻧﻔﺮ آزﻣﻮدﻧﯽ ﺗﻤﺮﯾﻦ ﮐﺮده ﺳﻦ 53/85±2/94 ﺳﺎل، ﺷﺎﺧﺺ ﺗﻮده ﺑﺪن 25/1±47/6 ﮐﯿﻠﻮﮔﺮم ﺑﺮ ﻣﺘﺮ ﻣﺮﺑﻊ، ﺣﺪاﮐﺜﺮ اﮐﺴﯿﮋن ﻣﺼﺮﻓﯽ 2/8 ± 42/38 ﻣﯿﻠﯽ ﻟﯿﺘﺮ ﺑﺮ ﮐﯿﻠﻮﮔﺮم در دﻗﯿﻘﻪ و 17 ﻧﻔﺮ ﺑﺪون ﺗﻤﺮﯾﻦ ﺳﻦ 2/83 ± 54/17 ﺳﺎل، ﺷﺎﺧﺺ ﺗﻮده ﺑﺪن 27/83±1/12 ﮐﯿﻠﻮﮔﺮم ﺑﺮ ﻣﺘﺮ ﻣﺮﺑﻊ ، ﺣﺪاﮐﺜﺮ اﮐﺴﯿﮋن ﻣﺼﺮﻓﯽ 31/86±1/67 ﻣﯿﻠﯽ ﻟﯿﺘﺮ ﺑﺮ ﮐﯿﻠﻮﮔﺮم در دﻗﯿﻘﻪ در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺷﺮﮐﺖ ﮐﺮدﻧﺪ. آزﻣﻮدﻧﯽ ﻫﺎ ﺗﺴﺖ اﺻﻼح ﺷﺪه ﺑﺮوس را ﺑﻪ ﻋﻨﻮان ﻣﺪﻟﯽ از ﺗﻤﺮﯾﻦ ﻓﺰاﯾﻨﺪه ورزﺷﯽ اﻧﺠﺎم دادﻧﺪ. ﻧﻤﻮﻧﻪ ﻫﺎی ﺧﻮﻧﯽ ﻗﺒﻞ، ﺑﻼﻓﺎﺻﻠﻪ ﺑﻌﺪ و ﯾﮏ ﺳﺎﻋﺖ ﭘﺲ از ﭘﺎﯾﺎن ﺗﺴﺖ درﯾﺎﻓﺖ ﺷﺪ. ﯾﺎﻓﺘﻪﻫﺎ: در ﻧﺘﺎﯾﺞ ﭘﯿﺶ آزﻣﻮن ﻣﯿﻠﻮﭘﺮاﮐﺴﯿﺪاز ﻫﺮ دو ﮔﺮوه ﺗﻔﺎوت ﻣﻌﻨﯽ داری وﺟﻮد ﻧﺪاﺷﺖ، اﻣﺎ اﯾﻦ ﻣﻘﺎدﯾﺮ ﺑﻼﻗﺎﺻﻠﻪ ﺑﻌﺪ و در دوره رﯾﮑﺎوری، در ﮔﺮوه ﺑﺪون ﺗﻤﺮﯾﻦ ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﺗﻤﺮﯾﻦ ﮐﺮده ﺗﻔﺎوت ﻣﻌﻨﯽ داری داﺷﺖ 0/05

acute response of myeloperoxidase and total oxidant status to progressive exercise training in trained and untrained middle-aged men

aim: neutrophils contain myeloperoxidase enzyme, which is involved in many cellular processes by producing oxidants and catalyzing hypochlorous acid. on the other hand, from middle age, physiological protection against oxidative stress starts to decrease. therefore, the main purpose of this study was to determine myeloperoxidase and total oxidative status changes in healthy trained middle aged men participating in regular recreational exercise training and untrained subjects.method: twenty trained age 53.85±2.94 years old, bmi 25.47±1.6 kg/m2, vo2max 42.38±2.8 ml.kg 1.min 1 and 17 untrained age 54.17±2.83 years old, bmi 27.83±1.12 kg/m2, vo2max 31.86±1.67 ml.kg 1.min 1 subjects participated in the study. subjects performed a modified bruce treadmill test as a model of progressive exercise training. blood samples were taken before t0 , immediately after t1 and one hour after the end of the test t2 .results: there was no significant change in pretest myeloperoxidase level in both groups, but these values were significantly changed in untrained group compared to the trained group at t1 and t2. in both groups, total oxidant status level significantly increased at t1 and decreased at t2 compared to the pretest.conclusion: regular participation in recreational exercise training can reduce the level of myeloperoxidase and total oxidant capacity of healthy middle aged men, and may be a proper way to reduce immune system and oxidative stress markers caused by aging in middle aged men.