Synthesis, Characterization and Spectroscopic Studies on the Interaction Between β-Casein and Co(II) Schiff Base Complex

In this study, co(ii) complex of a schiff base was synthesized and characterized. the in vitro binding behavior of this complex with β-casein (β-cn) was investigated using biophysical techniques. for evaluation the thermodynamics parameters of interactions between schiff base complex and β-cn, the fluorescence data at different temperatures were obtained. based on the results, the complex shows a significant binding affinity to β-cn and the process is enthalpy driven. the β-cn conformation was also changed through inducing a more unfolded structure. fluorescence resonance energy transfer was used to estimate the distance between donor (β-cn) and acceptor (complex). also, ligand and its complex were reacted with different bacteria (escherichia coli, bacillus subbtilis, staphylococcus aureus) to examin the inhibitory action of compounds. the results showed that the antimicrobial activity of metal complex is more than that of ligand. our results confirm the ability of β-cn as a protein carrier for co(ii) complex to deliver it to the target molecules.