Synergistic antibacterial effects of B-Chloro-L-alanine and phosphomycin on urinary tract isolates of E. coli

Introduction: the aim of this study was the evaluation of the synergistic effect of sub-mic doses of phosphomycin with b-chloro-l-alanine against urinary tract isolates of e. coli. materials and methods: a total of 40 isolates were collected from urine specimens submitted to the clinical diagnostic laboratories in urmia, iran. the amounts of mic and mbc for phosphomycin, b-chloro-l-alanine or a mixture of 0.5 mm b-chloro-l-alanine with sub-mic doses of phosphomycin were determined and three groups were compared. results: of 40 e. coli isolates, 12. 5% were susceptible to all investigated concentrations of phosphomycin and 2. 5% were resistant. the mean mic value for phosphomycin in the other e. coli isolates was determined as 25. 7± 35. 5 µg/ ml. all of the bacterial isolates were resistant to all investigated concentrations of b-chloro-l-alanine. application of b-chloro-l-alanine and phosphomycin combination decreased the mic and mbc values in 22. 5% of the isolates. discussion and conclusion: this study suggests that b-chloro-l-alanine and phosphomycin combination have in vitro synergistic effect on some isolates of urinary tract e. coli.