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Endothelial progenitor cells regenerate infracted myocardium with neovascularisation development
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نویسنده
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abd el aziz m.t. ,abd el nabi e.a. ,abd el hamid m. ,sabry d. ,atta h.m. ,rahed l.a. ,shamaa a. ,mahfouz s. ,taha f.m. ,elrefaay s. ,gharib d.m. ,elsetohy k.a.
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منبع
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journal of advanced research - 2015 - دوره : 6 - شماره : 2 - صفحه:133 -144
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چکیده
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We achieved possibility of isolation,characterization human umbilical cord blood endothelial progenitor cells (epcs),examination potency of epcs to form new blood vessels and differentiation into cardiomyoctes in canines with acute myocardial infarction (ami). epcs were separated and cultured from umbilical cord blood. their phenotypes were confirmed by uptake of double stains dioctadecyl tetramethylindocarbocyanine-labeled acetylated ldl and fitclabeled ulex europaeus agglutinin 1 (dildl-uea-1). epcs of cord blood were counted. human vegfr-2 and enos from the cultured epcs were assessed by qpcr. human epcs was transplanted intramyocardially in canines with ami. ecg and cardiac enzymes (ck-mb and troponin i) were measured to assess severity of cellular damage. histopathology was done to assess neovascularisation. immunostaining was done to detect epcs transdifferentiation into cardiomyocytes in peri-infarct cardiac tissue. qpcr for human genes (hvegfr-2,and enos) was done to assess homing and angiogenic function of transplanted epcs. cultured human cord blood exhibited an increased number of epcs and significant high expression of hvegfr-2 and enos genes in the culture cells. histopathology showed increased neovascularization and immunostaining showed presence of epcs newly differentiated into cardiomyocytelike cells. our findings suggested that hepcs can mediate angiogenesis and differentiate into cardiomyoctes in canines with ami. © 2014 production and hosting by elsevier b.v.
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کلیدواژه
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Acute myocardial infarction; Canine; Human EPCs; Neovascularization
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آدرس
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medical biochemistry and molecular biology department,faculty of medicine,cairo university, Egypt, medical biochemistry and molecular biology department,faculty of medicine,cairo university,cairo,egypt,clinical biochemistry department,faculty of medicine,king abdulaziz university, Saudi Arabia, cardiology department,faculty of medicine,cairo university, Egypt, medical biochemistry and molecular biology department,faculty of medicine,cairo university, Egypt, medical biochemistry and molecular biology department,faculty of medicine,cairo university,cairo,egypt,clinical biochemistry department,faculty of medicine,king abdulaziz university,rabigh branch, Saudi Arabia, medical biochemistry and molecular biology department,faculty of medicine,cairo university, Egypt, surgery department,faculty of veterinary medicine,cairo university, Egypt, pathology department,faculty of medicine,cairo university, Egypt, medical biochemistry and molecular biology department,faculty of medicine,cairo university, Egypt, nuclear medicine department,faculty of medicine,cairo university, Egypt, medical biochemistry and molecular biology department,faculty of medicine,cairo university, Egypt, obstetrics and gynaecology department,faculty of medicine,cairo university, Egypt
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Authors
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