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   The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis  
   
نویسنده jeong s.w. ,kim g.-s. ,lee w.s. ,kim y.-h. ,kang n.j. ,jin j.s. ,lee g.m. ,kim s.t. ,abd el-aty a.m. ,shim j.-h. ,shin s.c.
منبع journal of advanced research - 2015 - دوره : 6 - شماره : 3 - صفحه:493 -499
چکیده    The present study was conducted to characterize the polyphenolic contents of lettuce leaves grown under different night-time temperatures (4,12,and 20. °c) and cultivation durations (5,15,and 20. days) using high performance liquid chromatography-tandem mass spectrometry (lc/ms/ms). the assay method was validated based on specificity,linearity,accuracy,precision,and the performance limit. the total polyphenolic contents were highest (2462.6. mg/kg) after transplantation at a night temperature of 20. °c on day 20 and lowest (1132.7. mg/kg) at the same temperature on day 5. quantification and principal component analysis showed that the relative contents of quercetin and kaempferol were markedly higher during the early stage of cultivation (day 5) than those of day 15 and 20,and that night-time temperatures of 12 and 20. °c on day 20 were favorable for producing polyphenol-rich lettuce containing caffeic acid. in conclusion,a synergistic effect between high night-time temperatures (12 and 20. °c) and cultivation duration (20. days) produced lettuce rich in polyphenols compared to that at low temperature (4. °c). © 2015.
کلیدواژه Compositions; Lactuca sativa L.; Night growth temperature; Polyphenols; Principal component analysis
آدرس department of chemistry and research institute of life science,gyeongsang national university, South Korea, research institute of life science and college of veterinary medicine,gyeongsang national university, South Korea, department of internal medicine,institute of health sciences,gyeongsang national university, South Korea, department of chemistry and research institute of life science,gyeongsang national university, South Korea, department of horticulture and research institute of life science,gyeongsang national university, South Korea, korea basic science institute busan centre,division of high technology materials research,gangseo-gu, South Korea, department of information statistics,research institute of natural science,gyeongsang national university, South Korea, department of information statistics,research institute of natural science,gyeongsang national university, South Korea, biotechnology research institute,chonnam national university,77 yongbong-ro,buk-gu,gwangju,south korea,department of pharmacology,faculty of veterinary medicine,cairo university,giza,egypt,department of veterinary pharmacology and toxicology,college of veterinary medicine,konkuk university, South Korea, biotechnology research institute,chonnam national university,77 yongbong-ro,buk-gu, South Korea, department of chemistry and research institute of life science,gyeongsang national university, South Korea
 
     
   
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